ʻO ke kolamu Ghost-Sniper Column Chromasir HPLC UPLC kolamu e hoʻopau i nā piko ʻuhane
Hana ʻia ke kolamu Ghost-sniper e hoʻopau i nā piko ʻuhane. ʻAʻole ʻike ʻia ke kumu o nā piko ʻuhane i loko o kahi chromatogram, ʻike pinepine ʻia i ka wā o ke kaʻina o ka hoʻokaʻawale chromatographic i ka elution gradient a i ʻole ka hana lōʻihi. ʻO ka hiki ʻana mai o nā peak ghost he mana hohonu i nā hoʻokolohua a nā mea loiloi. Hana ʻia ke kolamu Ghost-sniper e hoʻoponopono i kēlā mau pilikia a hana i ka hana hoʻokolohua ʻoi aku ka maʻalahi a me ka maikaʻi. Hiki ke hoʻohana ʻia kēia kolamu ma kahi kūlana koʻikoʻi a hōʻike i kahi hopena hopu nui. He ala maikaʻi loa ia e hoʻopau ai i ke keʻakeʻa ʻana mai nā puʻupuʻu ʻuhane ma ke ʻano hōʻoia a me ka nānā ʻana i ka waiwai.
| Mahele No. | Anana | Volume | Palapala noi |
| MC5046091P | 50×4.6mm | ma kahi o 800ul | HPLC |
| MC3546092P | 35×4.6mm | ma kahi o 580ul | HPLC |
| MC5021093P | 50×2.1mm | ma kahi o 170ul | UPLC |
| MC3040096P | 30×4.0mm | ma kahi o 380ul | HPLC ka leo kolamu haahaa |
Hoʻokomo
Noi a me na hopena
1. Inā ho'onohonoho 'ia ka 'imi 'ana i loko o ka 'ōnaehana HPLC, pono e ho'onui i ka manawa koena hou no kahi o 5 min - 10 min i kou mau kūlana chromatographic, no ka mana o ka leo o ke kolamu ghost-sniper.
2. No nā kolamu hou, e holoi iā lākou me ka 100% acetonitrile ma ke kahe kahe o 0.5ml / min no nā hola 4 ma mua o ka hoʻohana ʻana.
3. Ion-pair reagents in mobile phase, hiki ke hoʻopili ʻia e ka ghost-sniper column, hiki ke hoʻololi i ka manawa paʻa a me ke ʻano kiʻekiʻe o kāu pahuhopu. E ʻoluʻolu e hoʻohana me ka akahele ma lalo o ia ʻano hana paʻa.
4. Aia ke ola o ke kolamu i nā kūlana analytical, e like me ka pae kelepona. ka maemae o ka solvent, a me ka haumia o na lako. E ʻoluʻolu e hoʻololi i ke kolamu ghost-sniper i nā manawa a pau e hōʻoia i ka hana.
5. Manaʻo ʻia e hoʻololi i ke kolamu ghost-sniper inā ʻoi aku ka maikaʻi o ka hopena o ka hopu ʻana a ʻaʻole hiki ke hoʻokō ʻia nā koi.
6. Ma ke ʻano hoʻomaʻemaʻe i ka chromatograph wai, hiki i ke kolamu ghost-sniper ke kānana i nā ʻāpana paʻa a hoʻopau i nā mea haumia ma mua o ka injector. Hāʻawi pū ka kolamu ghost-sniper i nā mea kani a me ke kolamu chromatographic me ka pale ʻoi aku ka maikaʻi, a hoʻomaikaʻi i ka chromatogram.
7. Inā loa'a ka pa'akai pa'akai ma mua o ka ho'ohana 'ana a ma hope o ka ho'ohana 'ana, e ho'ouna 'ia e holoi me 10% solution phase organic (10% methanol a i 'ole acetonitrile), i mea e pale aku ai i ka pa'akai pale a pale i ke kolamu.
8. E ʻoluʻolu e nānā ʻaʻole hiki ke hopu ʻia nā piko ʻuhane a pau e ke kolamu Ghost-Sniper.
9. Inā haʻalele ke kolamu no ka manawa lōʻihi, pono e mālama ʻia i loko o ka wai wai kūlohelohe (70% methanol a i ʻole acetonitrile). A e ʻoluʻolu e holoi iā ia me 100% acetonitrile ma ke kahe kahe o 0.5 ml / min no 1 hola ma mua o ka hoʻohana ʻana.
